68 lines
2.3 KiB
Markdown
68 lines
2.3 KiB
Markdown
# Marmoset Tracer-Injection [R01_0110]
|
|
|
|
Authors: Akiya Watakabe<sup>1</sup>*, Henrik Skibbe<sup>2</sup>
|
|
|
|
1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
|
|
2. Brain Image Analysis Unit, RIKEN Center for Brain Science
|
|
|
|
*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)
|
|
|
|
|
|
## DATASET DESCRIPTION
|
|
|
|
This dataset contains **TissueCyte** images produced during **tracer injection** experiment on Marmoset **R01_0110**
|
|
|
|
* Microscopy images and annotations: Akiya Watakabe.
|
|
* Image post-processing: Henrik Skibbe.
|
|
|
|
|
|
For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
|
|
|
|
|
|
## EXPERIMENT METADATA
|
|
|||
|
|
|----|-----|
|
|
|Brain/MINDS ID|R01_0110|
|
|
|Group|Yamamori|
|
|
|Lab identifier|TT77|
|
|
|Region of injection|A45|
|
|
|Side of injection|left|
|
|
|Summary|A45 injection. Small spread in A47L. Weak tracer signals. Projections to visual areas may suggest its position as FEF for small saccade. |
|
|
|Known issues|Expression is low. In particular, low expession in layer 6. Potential interference of AAV-smFP constructs.|
|
|
|Age|6.7|
|
|
|Sex|F|
|
|
|Body weight (g)|386|
|
|
|Date of birth|20120719|
|
|
|Injection date|20190219|
|
|
|Survival period (day)|27|
|
|
| Tracer **1** |(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml|
|
|
| Direction|anterograde|
|
|
| Method|0.2ul x 1 (@ 1.8 mm) 5min |
|
|
| Tracer **2** |AAV2retro-EF1-cre: 1.5x10e12 vg/ml|
|
|
| Injection Site|Coinjection with tracer 1|
|
|
| Direction|retro|
|
|
| Tracer **3** |10 % BDA|
|
|
| Injection Site|contra/MT|
|
|
| Direction|antero|
|
|
| Method|0.5µl/5min x2 depths|
|
|
| Tracer **4** |TET-smFP-myc (thy1-tTA 1/TRE-smFP-myc 1)x10e12 vg/ml|
|
|
| Injection Site|AP=0, R=6.5|
|
|
| Direction|antero|
|
|
| Method|0.1 µl/5 min @ 0.7 mm|
|
|
| Tracer **5** |TET-smFP-FLAG (thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml|
|
|
| Injection Site|AP=0, R=4.3|
|
|
| Direction|antero|
|
|
| Method|0.1 µl/5 min @ 0.7 mm|
|
|
| Tracer **6** |TET-smFP-HA (thy1-tTA 1/TRE-smFP-HA 1)x10e12 vg/ml|
|
|
| Injection Site|AP=0, L=4.3|
|
|
| Direction|antero|
|
|
| Method|0.1 µl/5 min @ 0.7 mm|
|
|
|
|
|
|
## CONTENT
|
|
|
|
| File name | Description |
|
|
| ---- | ---- |
|
|
|[`NN_tracer`](NN_tracer)/||
|
|
|[`c2_50mu`](c2_50mu)/||
|
|
|[`labels`](labels)/||
|