Marmoset Tracer-Injection [R01_0110]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0110

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0110
Group	Yamamori
Lab identifier	TT77
Region of injection	A45
Side of injection	left
Summary	A45 injection. Small spread in A47L. Weak tracer signals. Projections to visual areas may suggest its position as FEF for small saccade.
Known issues	Expression is low. In particular, low expession in layer 6. Potential interference of AAV-smFP constructs.
Age	6.7
Sex	F
Body weight (g)	386
Date of birth	20120719
Injection date	20190219
Survival period (day)	27
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.2ul x 1 (@ 1.8 mm) 5min
Tracer 2	AAV2retro-EF1-cre: 1.5x10e12 vg/ml
Injection Site	Coinjection with tracer 1
Direction	retro
Tracer 3	10 % BDA
Injection Site	contra/MT
Direction	antero
Method	0.5µl/5min x2 depths
Tracer 4	TET-smFP-myc (thy1-tTA 1/TRE-smFP-myc 1)x10e12 vg/ml
Injection Site	AP=0, R=6.5
Direction	antero
Method	0.1 µl/5 min @ 0.7 mm
Tracer 5	TET-smFP-FLAG (thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml
Injection Site	AP=0, R=4.3
Direction	antero
Method	0.1 µl/5 min @ 0.7 mm
Tracer 6	TET-smFP-HA (thy1-tTA 1/TRE-smFP-HA 1)x10e12 vg/ml
Injection Site	AP=0, L=4.3
Direction	antero
Method	0.1 µl/5 min @ 0.7 mm

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.