69 lines
2.3 KiB
Markdown
69 lines
2.3 KiB
Markdown
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# Marmoset Tracer-Injection [R01_0110]
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Authors: Akiya Watakabe<sup>1</sup>*, Henrik Skibbe<sup>2</sup>
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1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
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2. Brain Image Analysis Unit, RIKEN Center for Brain Science
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*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)
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## DATASET DESCRIPTION
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This dataset contains **TissueCyte** images produced during **tracer injection** experiment on Marmoset **R01_0110**
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* Microscopy images and annotations: Akiya Watakabe.
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* Image post-processing: Henrik Skibbe.
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For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
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## EXPERIMENT METADATA
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|----|-----|
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|Brain/MINDS ID|R01_0110|
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|Group|Yamamori|
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|Lab identifier|TT77|
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|Region of injection|A45|
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|Side of injection|left|
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|Summary|A45 injection. Small spread in A47L. Weak tracer signals. Projections to visual areas may suggest its position as FEF for small saccade. |
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|Known issues|Expression is low. In particular, low expession in layer 6. Potential interference of AAV-smFP constructs.|
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|Age|6.7|
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|Sex|F|
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|Body weight (g)|386|
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|Date of birth|20120719|
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|Injection date|20190219|
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|Survival period (day)|27|
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| Tracer **1** |(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml|
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| Direction|anterograde|
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| Method|0.2ul x 1 (@ 1.8 mm) 5min |
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| Tracer **2** |AAV2retro-EF1-cre: 1.5x10e12 vg/ml|
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| Injection Site|Coinjection with tracer 1|
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| Direction|retro|
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| Tracer **3** |10 % BDA|
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| Injection Site|contra/MT|
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| Direction|antero|
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| Method|0.5µl/5min x2 depths|
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| Tracer **4** |TET-smFP-myc (thy1-tTA 1/TRE-smFP-myc 1)x10e12 vg/ml|
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| Injection Site|AP=0, R=6.5|
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| Direction|antero|
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| Method|0.1 µl/5 min @ 0.7 mm|
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| Tracer **5** |TET-smFP-FLAG (thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml|
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| Injection Site|AP=0, R=4.3|
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| Direction|antero|
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| Method|0.1 µl/5 min @ 0.7 mm|
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| Tracer **6** |TET-smFP-HA (thy1-tTA 1/TRE-smFP-HA 1)x10e12 vg/ml|
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| Injection Site|AP=0, L=4.3|
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| Direction|antero|
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| Method|0.1 µl/5 min @ 0.7 mm|
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## CONTENT
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| File name | Description |
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| ---- | ---- |
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|[`NN_tracer`](NN_tracer)/||
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|[`c2_50mu`](c2_50mu)/||
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|[`labels`](labels)/||
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