1.8 KiB
1.8 KiB
Marmoset Tracer-Injection [R04_0080]
Authors: Akiya Watakabe1*, Henrik Skibbe2
- Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
- Brain Image Analysis Unit, RIKEN Center for Brain Science
*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)
DATASET DESCRIPTION
This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R04_0080
- Microscopy images and annotations: Akiya Watakabe.
- Image post-processing: Henrik Skibbe.
For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
EXPERIMENT METADATA
| Brain/MINDS ID | R04_0080 |
| Group | Yamamori |
| Lab identifier | TT58 |
| Region of injection | A10 |
| Side of injection | left |
| Summary | A10 injection; robust columnar projections within PFC and toward anterior temporal lobe. |
| Known issues | Tissue is damaged within amygdala and tracer segmentation is somewhat compromized in BLA. |
| Age | 3.2 |
| Sex | F |
| Body weight (g) | 266 |
| Date of birth | 20150220 |
| Injection date | 20180327 |
| Survival period (day) | 29 |
| Tracer 1 | (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml |
| Direction | anterograde |
| Method | 0.1ul x 2 (@ 0.8 and 1.2mm) 5min each |
| Tracer 2 | AAV2retro-EF1-cre: 0.8x10e13 vg/ml |
| Injection Site | contra/8aD |
| Direction | retro |
| Method | 0.2ul x 2 (@ 0.8 and 1.2mm) 5min each |
| Tracer 3 | 10 % BDA |
| Injection Site | contra/PCC |
| Direction | antero |
| Method | 0.5µl/5min x2 |
CONTENT
| File name | Description |
|---|---|
NN_tracer/ |
|
c2_50mu/ |
|
labels/ |
|
c1_50mu/ |
|
c3_50mu/ |
|
Backlit_slices/ |
|
Nissl_slices/ |