Marmoset Tracer-Injection [R04_0080]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R04_0080

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R04_0080
Group	Yamamori
Lab identifier	TT58
Region of injection	A10
Side of injection	left
Summary	A10 injection; robust columnar projections within PFC and toward anterior temporal lobe.
Known issues	Tissue is damaged within amygdala and tracer segmentation is somewhat compromized in BLA.
Age	3.2
Sex	F
Body weight (g)	266
Date of birth	20150220
Injection date	20180327
Survival period (day)	29
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.1ul x 2 (@ 0.8 and 1.2mm) 5min each
Tracer 2	AAV2retro-EF1-cre: 0.8x10e13 vg/ml
Injection Site	contra/8aD
Direction	retro
Method	0.2ul x 2 (@ 0.8 and 1.2mm) 5min each
Tracer 3	10 % BDA
Injection Site	contra/PCC
Direction	antero
Method	0.5µl/5min x2

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/