2.2 KiB
2.2 KiB
Marmoset Tracer-Injection [R04_0023]
Authors: Akiya Watakabe1*, Henrik Skibbe2
- Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
- Brain Image Analysis Unit, RIKEN Center for Brain Science
*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)
DATASET DESCRIPTION
This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R04_0023
- Microscopy images and annotations: Akiya Watakabe.
- Image post-processing: Henrik Skibbe.
For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
EXPERIMENT METADATA
| Brain/MINDS ID | R04_0023 |
| Lab identifier | TT83 |
| Region of injection | A47O |
| Summary | A47O injection with A47L and ProM trasnduction. ("vlPFC") Tracer signal is weak. |
| Known issues | Relatively weak signal. |
| Age | 5.2 |
| Sex | M |
| Body weight (g) | 376 |
| Date of birth | 20141225 |
| Injection date | 20200115 |
| Survival period (day) | 29 |
| Tracer 1 | (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml |
| Direction | anterograde |
| Method | 0.2ul x 1 5min |
| Tracer 2 | AAV2retroEF1-Cre 1.5x10e12 vg/ml |
| Injection Site | Coinjection with tracer 1 |
| Direction | retro |
| Tracer 3 | 10 % BDA |
| Injection Site | contra MD thalamus caudal |
| Direction | antero |
| Method | 0.2ul/5min |
| Tracer 4 | CamKII-smFP-myc (CamKII-smFP-myc 3.7x10e12 vg/ml) |
| Injection Site | ipsi/Opro |
| Direction | antero |
| Method | 0.2 µl/5 min @ 0.7 mm |
| Tracer 5 | (thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml |
| Injection Site | contra MD thalamus rostral |
| Direction | antero |
| Method | 0.2ul/5min |
| Tracer 6 | AAV2retro-smFP-HA.H2B(AAV2retro-CAG-smFP-HA.H2B 3.7x10e12 vg/ml) |
| Injection Site | Coinjection with tracer 4 |
| Direction | retro |
CONTENT
| File name | Description |
|---|---|
NN_tracer/ |
|
c2_50mu/ |
|
labels/ |
|
c1_50mu/ |
|
c3_50mu/ |
|
Backlit_slices/ |
|
Nissl_slices/ |