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Marmoset Tracer Injection – Image set [MTI-R04_0023]
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README.md

Marmoset Tracer-Injection [R04_0023]

Authors: Akiya Watakabe1*, Henrik Skibbe2

  1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
  2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R04_0023

  • Microscopy images and annotations: Akiya Watakabe.
  • Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA
Brain/MINDS ID R04_0023
Lab identifier TT83
Region of injection A47O
Summary A47O injection with A47L and ProM trasnduction. ("vlPFC") Tracer signal is weak.
Known issues Relatively weak signal.
Age 5.2
Sex M
Body weight (g) 376
Date of birth 20141225
Injection date 20200115
Survival period (day) 29
 Tracer 1 (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
 Direction anterograde
 Method 0.2ul x 1 5min
 Tracer 2 AAV2retroEF1-Cre 1.5x10e12 vg/ml
 Injection Site Coinjection with tracer 1
 Direction retro
 Tracer 3 10 % BDA
 Injection Site contra MD thalamus caudal
 Direction antero
 Method 0.2ul/5min
 Tracer 4 CamKII-smFP-myc (CamKII-smFP-myc 3.7x10e12 vg/ml)
 Injection Site ipsi/Opro
 Direction antero
 Method 0.2 µl/5 min @ 0.7 mm
 Tracer 5 (thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml
 Injection Site contra MD thalamus rostral
 Direction antero
 Method 0.2ul/5min
 Tracer 6 AAV2retro-smFP-HA.H2B(AAV2retro-CAG-smFP-HA.H2B 3.7x10e12 vg/ml)
 Injection Site Coinjection with tracer 4
 Direction retro

CONTENT

File name Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/