MTI-R01_0112/README.md

Marmoset Tracer-Injection [R01_0112]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0112

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0112
Group	Yamamori
Lab identifier	TT80
Region of injection	A32
Side of injection	left
Summary	A32 and A9 injection.
Known issues	Almost split between A32 and A9. Low layer 6 labeling.
Age	6
Sex	F
Date of birth	20130812
Injection date	20190731
Survival period (day)	28
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.2ul x 1 (@ 6.4 mm) 5min
Tracer 2	AAV2retro-EF1-cre: 1.5x10e12 vg/ml
Injection Site	Coinjection with tracer 1
Direction	retro
Tracer 3	10 % BDA
Injection Site	contra/A10
Direction	antero
Method	0.2µl/5min x1
Tracer 4	TET-smFP-myc (thy1-tTA 1/TRE-smFP-myc 1)x10e12 vg/ml
Injection Site	contra/A14
Direction	antero
Method	0.2 µl/5 min @ 0.7 mm
Tracer 5	TET-smFP-FLAG (thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml
Injection Site	contra/A14
Direction	antero
Method	0.2 µl/5 min @ 0.7 mm
Comment	slightly different from tracer 4, but mixed
Tracer 6	AAV2retro-smFP-HA.H2B(AAV2retro-CAG-smFP-HA.H2B 5x10e12 vg/ml)
Injection Site	coinjected with tracer 4
Direction	retro

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.