2021-12-27 00:00:00 +09:00
# Marmoset Tracer-Injection [R01_0112]
Authors: Akiya Watakabe< sup > 1< / sup > *, Henrik Skibbe< sup > 2< / sup >
1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science
*corresponding authors: Akiya Watakabe (akiya.watakabe@riken .jp)
## DATASET DESCRIPTION
This dataset contains **TissueCyte** images produced during **tracer injection** experiment on Marmoset **R01_0112**
* Microscopy images and annotations: Akiya Watakabe.
* Image post-processing: Henrik Skibbe.
For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
## EXPERIMENT METADATA
|||
|----|-----|
|Brain/MINDS ID|R01_0112|
|Group|Yamamori|
|Lab identifier|TT80|
|Region of injection|A32|
|Side of injection|left|
|Summary|A32 and A9 injection. |
|Known issues|Almost split between A32 and A9. Low layer 6 labeling. |
|Age|6|
|Sex|F|
|Date of birth|20130812|
|Injection date|20190731|
|Survival period (day)|28|
|& #x00A0 ; Tracer **1** |(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml|
|& #x00A0 ; Direction|anterograde|
|& #x00A0 ; Method|0.2ul x 1 (@ 6.4 mm) 5min |
|& #x00A0 ; Tracer **2** |AAV2retro-EF1-cre: 1.5x10e12 vg/ml|
|& #x00A0 ; Injection Site|Coinjection with tracer 1|
|& #x00A0 ; Direction|retro|
|& #x00A0 ; Tracer **3** |10 % BDA|
|& #x00A0 ; Injection Site|contra/A10|
|& #x00A0 ; Direction|antero|
|& #x00A0 ; Method|0.2µl/5min x1|
|& #x00A0 ; Tracer **4** |TET-smFP-myc (thy1-tTA 1/TRE-smFP-myc 1)x10e12 vg/ml|
|& #x00A0 ; Injection Site|contra/A14|
|& #x00A0 ; Direction|antero|
|& #x00A0 ; Method|0.2 µl/5 min @ 0.7 mm|
|& #x00A0 ; Tracer **5** |TET-smFP-FLAG (thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml|
|& #x00A0 ; Injection Site|contra/A14|
|& #x00A0 ; Direction|antero|
|& #x00A0 ; Method|0.2 µl/5 min @ 0.7 mm|
|& #x00A0 ; Comment|slightly different from tracer 4, but mixed|
|& #x00A0 ; Tracer **6** |AAV2retro-smFP-HA.H2B(AAV2retro-CAG-smFP-HA.H2B 5x10e12 vg/ml)|
|& #x00A0 ; Injection Site|coinjected with tracer 4|
|& #x00A0 ; Direction|retro|
## CONTENT
| File name | Description |
| ---- | ---- |
|[`NN_tracer` ](NN_tracer )/||
|[`c2_50mu` ](c2_50mu )/||
|[`labels` ](labels )/||
2023-08-17 00:00:00 +09:00
|[`c1_50mu` ](c1_50mu )/||
|[`c3_50mu` ](c3_50mu )/||
|[`Backlit_slices` ](Backlit_slices )/||
|[`Nissl_slices` ](Nissl_slices )/||
2023-09-14 00:00:00 +09:00
**Note:** Color lookup table used in [atlas region labels slices ](labels ) can be found [here ](https://cau-gin.brainminds.riken.jp/brainminds/BMA-2017/src/master/bma-1-clut.ctbl ).
