1.6 KiB
1.6 KiB
Marmoset Tracer-Injection [R01_0070]
Authors: Akiya Watakabe1*, Henrik Skibbe2
- Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
- Brain Image Analysis Unit, RIKEN Center for Brain Science
*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)
DATASET DESCRIPTION
This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0070
- Microscopy images and annotations: Akiya Watakabe.
- Image post-processing: Henrik Skibbe.
For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
EXPERIMENT METADATA
| Brain/MINDS ID | R01_0070 |
| Group | Yamamori |
| Lab identifier | TT45 |
| Region of injection | A8aV |
| Side of injection | left |
| Summary | A8aV/A6Va injection. Weak tracer signals. Problems in injection spread. |
| Known issues | Layer 6 neurons are not labeled well. Additional labeling was observed in layer 2/3 in area 8c/6Va. Obviously low projection to MD in comparison with labeling in IC(?). |
| Age | 6.6 |
| Sex | F |
| Body weight (g) | 339 |
| Date of birth | 20101226 |
| Injection date | 20170613 |
| Survival period (day) | 30 |
| Tracer 1 | (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml |
| Direction | anterograde |
| Method | 0.1ul x 2 (@ 0.8 and 1.2mm) 5min each |
| Tracer 2 | 10 % BDA |
| Injection Site | contra/premotor |
| Direction | antero |
| Method | 0.5ul/5minx2 |
CONTENT
| File name | Description |
|---|---|
NN_tracer/ |
|
c2_50mu/ |
|
labels/ |