Marmoset Tracer-Injection [R01_0070]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0070

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0070
Group	Yamamori
Lab identifier	TT45
Region of injection	A8aV
Side of injection	left
Summary	A8aV/A6Va injection. Weak tracer signals. Problems in injection spread.
Known issues	Layer 6 neurons are not labeled well. Additional labeling was observed in layer 2/3 in area 8c/6Va. Obviously low projection to MD in comparison with labeling in IC(?).
Age	6.6
Sex	F
Body weight (g)	339
Date of birth	20101226
Injection date	20170613
Survival period (day)	30
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.1ul x 2 (@ 0.8 and 1.2mm) 5min each
Tracer 2	10 % BDA
Injection Site	contra/premotor
Direction	antero
Method	0.5ul/5minx2

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.