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Marmoset Tracer Injection – Image set [MTI-R04_0095]
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README.md

Marmoset Tracer-Injection [R04_0095]

Authors: Akiya Watakabe1*, Henrik Skibbe2

  1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
  2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R04_0095

  • Microscopy images and annotations: Akiya Watakabe.
  • Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA
Brain/MINDS ID R04_0095
Lab identifier TT65
Region of injection A8b
Summary A8b injection ("med").
Age 2.6
Sex F
Body weight (g) 330
Date of birth 20160123
Injection date 20180710
Survival period (day) 28
 Tracer 1 (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
 Direction anterograde
 Method 0.1ul x 2 (@ 0.8 and 1.2mm) 5min each
 Tracer 2 AAV2retro-EF1-cre: 1.5x10e12 vg/ml
 Injection Site Coinjection with tracer 1
 Direction retro
 Tracer 3 10 % BDA
 Injection Site contra-injection
 Direction antero
 Method 0.25µl/5min x2

CONTENT

File name Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.