58 lines
1.7 KiB
Markdown
58 lines
1.7 KiB
Markdown
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# Marmoset Tracer-Injection [R04_0080]
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Authors: Akiya Watakabe<sup>1</sup>*, Henrik Skibbe<sup>2</sup>
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1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
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2. Brain Image Analysis Unit, RIKEN Center for Brain Science
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*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)
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## DATASET DESCRIPTION
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This dataset contains **TissueCyte** images produced during **tracer injection** experiment on Marmoset **R04_0080**
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* Microscopy images and annotations: Akiya Watakabe.
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* Image post-processing: Henrik Skibbe.
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For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
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## EXPERIMENT METADATA
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|----|-----|
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|Brain/MINDS ID|R04_0080|
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|Group|Yamamori|
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|Lab identifier|TT58|
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|Region of injection|A10|
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|Side of injection|left|
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|Summary|A10 injection; robust columnar projections within PFC and toward anterior temporal lobe. |
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|Known issues|Tissue is damaged within amygdala and tracer segmentation is somewhat compromized in BLA.|
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|Age|3.2|
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|Sex|F|
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|Body weight (g)|266|
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|Date of birth|20150220|
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|Injection date|20180327|
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|Survival period (day)|29|
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| Tracer **1** |(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml|
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| Direction|anterograde|
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| Method|0.1ul x 2 (@ 0.8 and 1.2mm) 5min each|
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| Tracer **2** |AAV2retro-EF1-cre: 0.8x10e13 vg/ml|
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| Injection Site|contra/8aD|
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| Direction|retro|
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| Method|0.2ul x 2 (@ 0.8 and 1.2mm) 5min each|
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| Tracer **3** |10 % BDA|
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| Injection Site|contra/PCC|
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| Direction|antero|
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| Method|0.5µl/5min x2|
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## CONTENT
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| File name | Description |
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| ---- | ---- |
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|[`NN_tracer`](NN_tracer)/||
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|[`c2_50mu`](c2_50mu)/||
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|[`labels`](labels)/||
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