Marmoset Tracer-Injection [R01_0115]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0115

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0115
Group	Yamamori
Lab identifier	TT82
Region of injection	A32
Summary	At the border between A24b, A8b and A32. ("dACC") Conspicuous bilateral projections.
Age	8.4
Sex	F
Body weight (g)	418
Date of birth	20110616
Injection date	20191018
Survival period (day)	28
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.2ul x 1 (@ 3 mm) 5min
Tracer 2	AAV2retro-EF1-cre: 1.5x10e12 vg/ml
Injection Site	Coinjection with tracer 1
Direction	retro
Tracer 3	10 % BDA
Injection Site	ipsi/A32(next to tracer1)
Direction	antero
Method	0.2µl/5min x1
Tracer 4	CamKII-smFP-myc (CamKII-smFP-myc 3.7x10e12 vg/ml)
Injection Site	contra/A32
Direction	antero
Method	0.2 µl/5 min @ 0.7 mm
Tracer 5	(thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml
Injection Site	contra MD thalamus
Direction	antero
Method	0.2ul/5min
Tracer 6	AAV2retro-smFP-HA.H2B(AAV2retro-CAG-smFP-HA.H2B 3.7x10e12 vg/ml)
Injection Site	coinjected with tracer 4
Direction	retro

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/