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Marmoset Tracer Injection – Image set [MTI-R01_0115]
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README.md

Marmoset Tracer-Injection [R01_0115]

Authors: Akiya Watakabe1*, Henrik Skibbe2

  1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
  2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0115

  • Microscopy images and annotations: Akiya Watakabe.
  • Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA
Brain/MINDS ID R01_0115
Group Yamamori
Lab identifier TT82
Region of injection A32
Summary At the border between A24b, A8b and A32. ("dACC") Conspicuous bilateral projections.
Age 8.4
Sex F
Body weight (g) 418
Date of birth 20110616
Injection date 20191018
Survival period (day) 28
 Tracer 1 (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
 Direction anterograde
 Method 0.2ul x 1 (@ 3 mm) 5min
 Tracer 2 AAV2retro-EF1-cre: 1.5x10e12 vg/ml
 Injection Site Coinjection with tracer 1
 Direction retro
 Tracer 3 10 % BDA
 Injection Site ipsi/A32(next to tracer1)
 Direction antero
 Method 0.2µl/5min x1
 Tracer 4 CamKII-smFP-myc (CamKII-smFP-myc 3.7x10e12 vg/ml)
 Injection Site contra/A32
 Direction antero
 Method 0.2 µl/5 min @ 0.7 mm
 Tracer 5 (thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml
 Injection Site contra MD thalamus
 Direction antero
 Method 0.2ul/5min
 Tracer 6 AAV2retro-smFP-HA.H2B(AAV2retro-CAG-smFP-HA.H2B 3.7x10e12 vg/ml)
 Injection Site coinjected with tracer 4
 Direction retro

CONTENT

File name Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/