Marmoset Tracer-Injection [R01_0114]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0114

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0114
Group	Yamamori
Lab identifier	TT81
Region of injection	A25
Side of injection	left
Summary	A25 injection (+ 14C); robust basal forebrain, amygdala and temporal cortex projections.
Known issues	Low tissue background signal. May contain some AAV spread into the septum. Labels can be confirmed in fimbria and stria terminalis.
Age	9.8
Sex	F
Body weight (g)	338
Date of birth	20100126
Injection date	20190913
Survival period (day)	28
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.2ul x 1 (@ 7.4 mm) 5min
Tracer 2	AAV2retro-EF1-cre: 1.5x10e12 vg/ml
Injection Site	Coinjection with tracer 1
Direction	retro
Tracer 3	10 % BDA
Injection Site	contra/A10
Direction	antero
Method	0.2µl/5min x1
Tracer 4	CamKII-smFP-myc (CamKII-smFP-myc 3.7x10e12 vg/ml)
Injection Site	contra/A24bc (leak to A14)
Direction	antero
Method	0.2 µl/5 min @ 0.7 mm
Tracer 5	TET-smFP-FLAG (thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml
Injection Site	contra/A24 (leak to A14)
Direction	antero
Method	0.2ul x 1 5min
Tracer 6	AAV2retro-smFP-HA.H2B(AAV2retro-CAG-smFP-HA.H2B 3.7x10e12 vg/ml)
Injection Site	coinjected with tracer 4
Direction	retro

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/