MTI-R01_0107/README.md

Marmoset Tracer-Injection [R01_0107]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0107

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0107
Group	Yamamori
Lab identifier	TT78
Region of injection	A13L
Side of injection	left
Summary	A13L injection. ("OFC"). No leak in the dlPFC, shows typical OFC pattern. Physical damage around the injection site.
Known issues	Physical damage to the tissue around section 112. In particular, the injection site is severed from the medial side.
Age	2.5
Sex	M
Body weight (g)	327
Date of birth	20161006
Injection date	20190329
Survival period (day)	27
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.2ul x 1 (@ 1 mm above the "floor bone") 5min
Tracer 2	AAV2retro-EF1-cre: 1.5x10e12 vg/ml
Injection Site	Coinjection with tracer 1
Direction	retro
Tracer 3	10 % BDA
Injection Site	ipsi/A47O
Direction	antero
Method	0.2µl/5min x1
Tracer 4	TET-smFP-V5(thy1-tTA 1/TRE-smFP-V5 1)x10e12 vg/ml
Injection Site	AP=0, L=3
Direction	antero
Method	0.1 µl/5 min @ 0.7 mm
Tracer 5	TET-smFP-FLAG (thy1-tTA 1/TRE-smFP-FLAG 1)x10e12 vg/ml
Injection Site	AP=0, R=3
Direction	antero
Method	0.1 µl/5 min @ 0.7 mm
Tracer 6	TET-smFP-HA (thy1-tTA 1/TRE-smFP-HA 1)x10e12 vg/ml
Injection Site	contra/A25
Direction	antero
Method	0.2 µl/5 min @ 0.7 mm

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.