MTI-R01_0098/README.md
2023-05-09 00:00:00 +09:00

1.7 KiB

Marmoset Tracer-Injection [R01_0098]

Authors: Akiya Watakabe1*, Henrik Skibbe2

  1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
  2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0098

  • Microscopy images and annotations: Akiya Watakabe.
  • Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID R01_0098
Group Yamamori
Lab identifier TT73
Region of injection A24b
Side of injection left
Summary A24b/c injection. No A24a label. Diffuse and widespread layer 1 innervation in the cingular and insular areas.
Known issues Labeling along the needle track. A tissue fragment containing the injeection site is displaced into the midline around section 190.
Age 3.3
Sex M
Body weight (g) 553
Date of birth 20151004
Injection date 20181218
Survival period (day) 30
 Tracer 1 (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
 Direction anterograde
 Method 0.2ul x 1 (@ 3.1 mm) 5min
 Tracer 2 AAV2retro-EF1-cre: 1.5x10e12 vg/ml
 Injection Site Coinjection with tracer 1
 Direction retro
 Tracer 3 10 % BDA
 Injection Site contra-injection
 Direction antero
 Method 0.2µl/5min x1

CONTENT

File name Description
NN_tracer/
c2_50mu/
labels/