2021-12-27 00:00:00 +09:00
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# Marmoset Tracer-Injection [R01_0095]
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Authors: Akiya Watakabe<sup>1</sup>*, Henrik Skibbe<sup>2</sup>
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1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
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2. Brain Image Analysis Unit, RIKEN Center for Brain Science
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*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)
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## DATASET DESCRIPTION
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This dataset contains **TissueCyte** images produced during **tracer injection** experiment on Marmoset **R01_0095**
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* Microscopy images and annotations: Akiya Watakabe.
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* Image post-processing: Henrik Skibbe.
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For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
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## EXPERIMENT METADATA
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|----|-----|
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|Brain/MINDS ID|R01_0095|
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|Group|Yamamori|
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|Lab identifier|TT69|
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|Region of injection|A14R|
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|Side of injection|left|
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|Summary|A14R injection. ("mPFC") The tracer spread wide along the needle track. Robust anterior temporal cortex. |
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|Known issues|Injection into A14R. Labeling is robust. But strongly labels the adjascent A32 along the needle track. A32v and A13b as well. |
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|Age|2.3|
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|Sex|M|
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|Body weight (g)|290|
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|Date of birth|20160701|
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|Injection date|20180919|
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|Survival period (day)|28|
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| Tracer **1** |(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml|
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| Direction|anterograde|
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| Method|0.2ul x 1 (@ 1 mm above the "floor bone") 5min |
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| Tracer **2** |AAV2retro-EF1-cre: 1.5x10e12 vg/ml|
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| Injection Site|Coinjection with tracer 1|
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| Direction|retro|
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| Tracer **3** |10 % BDA|
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| Injection Site|contra-injection|
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| Direction|antero|
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| Method|0.2µl/5min x1|
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## CONTENT
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| File name | Description |
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| ---- | ---- |
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|[`NN_tracer`](NN_tracer)/||
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|[`c2_50mu`](c2_50mu)/||
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|[`labels`](labels)/||
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2023-08-17 00:00:00 +09:00
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|[`c1_50mu`](c1_50mu)/||
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|[`c3_50mu`](c3_50mu)/||
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|[`Backlit_slices`](Backlit_slices)/||
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|[`Nissl_slices`](Nissl_slices)/||
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