Marmoset Tracer-Injection [R01_0095]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0095

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0095
Group	Yamamori
Lab identifier	TT69
Region of injection	A14R
Side of injection	left
Summary	A14R injection. ("mPFC") The tracer spread wide along the needle track. Robust anterior temporal cortex.
Known issues	Injection into A14R. Labeling is robust. But strongly labels the adjascent A32 along the needle track. A32v and A13b as well.
Age	2.3
Sex	M
Body weight (g)	290
Date of birth	20160701
Injection date	20180919
Survival period (day)	28
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.2ul x 1 (@ 1 mm above the "floor bone") 5min
Tracer 2	AAV2retro-EF1-cre: 1.5x10e12 vg/ml
Injection Site	Coinjection with tracer 1
Direction	retro
Tracer 3	10 % BDA
Injection Site	contra-injection
Direction	antero
Method	0.2µl/5min x1

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/