2021-12-27 00:00:00 +09:00
# Marmoset Tracer-Injection [R01_0095]
Authors: Akiya Watakabe< sup > 1< / sup > *, Henrik Skibbe< sup > 2< / sup >
1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science
*corresponding authors: Akiya Watakabe (akiya.watakabe@riken .jp)
## DATASET DESCRIPTION
This dataset contains **TissueCyte** images produced during **tracer injection** experiment on Marmoset **R01_0095**
* Microscopy images and annotations: Akiya Watakabe.
* Image post-processing: Henrik Skibbe.
For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
## EXPERIMENT METADATA
|||
|----|-----|
|Brain/MINDS ID|R01_0095|
|Group|Yamamori|
|Lab identifier|TT69|
|Region of injection|A14R|
|Side of injection|left|
|Summary|A14R injection. ("mPFC") The tracer spread wide along the needle track. Robust anterior temporal cortex. |
|Known issues|Injection into A14R. Labeling is robust. But strongly labels the adjascent A32 along the needle track. A32v and A13b as well. |
|Age|2.3|
|Sex|M|
|Body weight (g)|290|
|Date of birth|20160701|
|Injection date|20180919|
|Survival period (day)|28|
|& #x00A0 ; Tracer **1** |(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml|
|& #x00A0 ; Direction|anterograde|
|& #x00A0 ; Method|0.2ul x 1 (@ 1 mm above the "floor bone") 5min |
|& #x00A0 ; Tracer **2** |AAV2retro-EF1-cre: 1.5x10e12 vg/ml|
|& #x00A0 ; Injection Site|Coinjection with tracer 1|
|& #x00A0 ; Direction|retro|
|& #x00A0 ; Tracer **3** |10 % BDA|
|& #x00A0 ; Injection Site|contra-injection|
|& #x00A0 ; Direction|antero|
|& #x00A0 ; Method|0.2µl/5min x1|
## CONTENT
| File name | Description |
| ---- | ---- |
|[`NN_tracer` ](NN_tracer )/||
|[`c2_50mu` ](c2_50mu )/||
|[`labels` ](labels )/||
2023-08-17 00:00:00 +09:00
|[`c1_50mu` ](c1_50mu )/||
|[`c3_50mu` ](c3_50mu )/||
|[`Backlit_slices` ](Backlit_slices )/||
|[`Nissl_slices` ](Nissl_slices )/||
2023-09-14 00:00:00 +09:00
**Note:** Color lookup table used in [atlas region labels slices ](labels ) can be found [here ](https://cau-gin.brainminds.riken.jp/brainminds/BMA-2017/src/master/bma-1-clut.ctbl ).
