1.9 KiB
1.9 KiB
Marmoset Tracer-Injection [R01_0092]
Authors: Akiya Watakabe1*, Henrik Skibbe2
- Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
- Brain Image Analysis Unit, RIKEN Center for Brain Science
*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)
DATASET DESCRIPTION
This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0092
- Microscopy images and annotations: Akiya Watakabe.
- Image post-processing: Henrik Skibbe.
For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
EXPERIMENT METADATA
| Brain/MINDS ID | R01_0092 |
| Group | Yamamori |
| Lab identifier | TT64 |
| Region of injection | A24a |
| Side of injection | left |
| Summary | A24a injection ("mPFC"). Leak into Cd. No label in A24b. |
| Known issues | Injection into narrow A24a. No leak into above-lying A24b. But small leak into the striatum (labeling of caudate cells lead to unusual labeling of striate-to SN pathway.) IG (indusium griseum) may be strogly labeled which may innervate septum. |
| Age | 2.4 |
| Sex | M |
| Body weight (g) | 305 |
| Date of birth | 20160211 |
| Injection date | 20180703 |
| Survival period (day) | 27 |
| Tracer 1 | (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml |
| Direction | anterograde |
| Method | 0.2ul x 1 (@ 4.3 mm) 5min |
| Tracer 2 | AAV2retro-EF1-cre: 1.5x10e12 vg/ml |
| Injection Site | Coinjection with tracer 1 |
| Direction | retro |
| Tracer 3 | 10 % BDA |
| Injection Site | contra-injection |
| Direction | antero |
| Method | 0.5µl/5min x1 |
CONTENT
| File name | Description |
|---|---|
NN_tracer/ |
|
c2_50mu/ |
|
labels/ |
|
c1_50mu/ |
|
c3_50mu/ |
|
Backlit_slices/ |
|
Nissl_slices/ |