2 KiB
2 KiB
Marmoset Tracer-Injection [R01_0090]
Authors: Akiya Watakabe1*, Henrik Skibbe2
- Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
- Brain Image Analysis Unit, RIKEN Center for Brain Science
*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)
DATASET DESCRIPTION
This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0090
- Microscopy images and annotations: Akiya Watakabe.
- Image post-processing: Henrik Skibbe.
For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
EXPERIMENT METADATA
| Brain/MINDS ID | R01_0090 |
| Group | Yamamori |
| Lab identifier | TT67 |
| Region of injection | A13M |
| Side of injection | left |
| Summary | A13M and A13L injection ("OFC"). Some leak. |
| Known issues | More injection in deeper layers. There is a small leak of injection to the head of the caudate and the above-lying A8b. |
| Age | 2.9 |
| Sex | F |
| Body weight (g) | 310 |
| Date of birth | 20151111 |
| Injection date | 20180904 |
| Survival period (day) | 27 |
| Tracer 1 | (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml |
| Direction | anterograde |
| Method | 0.2ul x 1 (@ 1 mm above the "floor bone") 5min |
| Tracer 2 | AAV2retro-EF1-cre: 1.5x10e12 vg/ml |
| Injection Site | Coinjection with tracer 1 |
| Direction | retro |
| Tracer 3 | 10 % BDA |
| Injection Site | ipsi/A11 |
| Direction | antero |
| Method | 0.5µl/5min x1 |
CONTENT
| File name | Description |
|---|---|
NN_tracer/ |
|
c2_50mu/ |
|
labels/ |
|
c1_50mu/ |
|
c3_50mu/ |
|
Backlit_slices/ |
|
Nissl_slices/ |
Note: Color lookup table used in atlas region labels slices can be found here.