Marmoset Tracer-Injection [R01_0078]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0078

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0078
Group	Yamamori
Lab identifier	TT52
Region of injection	A13L
Side of injection	left
Summary	A13L injection. ("OFC')Some leak in the dorsal areas.
Known issues	There exists considerable leak around A8b/A8aD border along the needle track.
Age	8.9
Sex	F
Body weight (g)	354
Date of birth	20090131
Injection date	20171120
Survival period (day)	29
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.2ul x 1 (@ 0.5mm above the "floor bone") 5min
Tracer 2	10 % BDA
Injection Site	contra/A24
Direction	antero
Method	1ul/10 min

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.