MTI-R01_0075/README.md
2023-09-14 00:00:00 +09:00

1.8 KiB

Marmoset Tracer-Injection [R01_0075]

Authors: Akiya Watakabe1*, Henrik Skibbe2

  1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
  2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0075

  • Microscopy images and annotations: Akiya Watakabe.
  • Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID R01_0075
Group Yamamori
Lab identifier TT46
Region of injection A8aV
Side of injection left
Summary A8aV injection. Widespread in layer 2.
Known issues Injection spread toward caudal direction in layer 2, covering large areas within A8aV.
Age 5.6
Sex F
Date of birth 20111206
Injection date 20170621
Survival period (day) 29
 Tracer 1 (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
 Direction anterograde
 Method 0.1ul x 2 (@ 0.8 and 1.2mm) 5min each
 Tracer 2 10 % BDA
 Injection Site contra/injection
 Direction antero
 Method 0.5ul/5minx2

CONTENT

File name Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.