54 lines
1.5 KiB
Markdown
54 lines
1.5 KiB
Markdown
|
# Marmoset Tracer-Injection [R01_0061]
|
||
|
|
||
|
Authors: Akiya Watakabe<sup>1</sup>*, Henrik Skibbe<sup>2</sup>
|
||
|
|
||
|
1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
|
||
|
2. Brain Image Analysis Unit, RIKEN Center for Brain Science
|
||
|
|
||
|
*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)
|
||
|
|
||
|
|
||
|
## DATASET DESCRIPTION
|
||
|
|
||
|
This dataset contains **TissueCyte** images produced during **tracer injection** experiment on Marmoset **R01_0061**
|
||
|
|
||
|
* Microscopy images and annotations: Akiya Watakabe.
|
||
|
* Image post-processing: Henrik Skibbe.
|
||
|
|
||
|
|
||
|
For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection
|
||
|
|
||
|
|
||
|
## EXPERIMENT METADATA
|
||
|
|||
|
||
|
|----|-----|
|
||
|
|Brain/MINDS ID|R01_0061|
|
||
|
|Group|Yamamori|
|
||
|
|Lab identifier|TT40|
|
||
|
|Region of injection|A8b|
|
||
|
|Side of injection|left|
|
||
|
|Summary|A8b injection with A6M leak. Columnar projections are rare. Robust deep layer projection in PCC. |
|
||
|
|Known issues|Targeted to A8b, but leaked into A6M. Injection was almost restricted to deep layers.|
|
||
|
|Age|8.5|
|
||
|
|Sex|M|
|
||
|
|Body weight (g)|386|
|
||
|
|Date of birth|20081117|
|
||
|
|Injection date|20170412|
|
||
|
|Survival period (day)|27|
|
||
|
| Tracer **1** |(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml|
|
||
|
| Direction|anterograde|
|
||
|
| Method|0.1ul x 2 (@ 0.8 and 1.2mm) 5min each|
|
||
|
| Tracer **2** |10 % BDA|
|
||
|
| Injection Site|contra/cingular|
|
||
|
| Direction|antero|
|
||
|
| Method|1ul/10 min|
|
||
|
|
||
|
|
||
|
## CONTENT
|
||
|
|
||
|
| File name | Description |
|
||
|
| ---- | ---- |
|
||
|
|[`NN_tracer`](NN_tracer)/||
|
||
|
|[`c2_50mu`](c2_50mu)/||
|
||
|
|[`labels`](labels)/||
|