Marmoset Tracer Injection – Image set [MTI-R01_0061]
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Marmoset Tracer-Injection [R01_0061]

Authors: Akiya Watakabe1*, Henrik Skibbe2

  1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
  2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0061

  • Microscopy images and annotations: Akiya Watakabe.
  • Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID R01_0061
Group Yamamori
Lab identifier TT40
Region of injection A8b
Side of injection left
Summary A8b injection with A6M leak. Columnar projections are rare. Robust deep layer projection in PCC.
Known issues Targeted to A8b, but leaked into A6M. Injection was almost restricted to deep layers.
Age 8.5
Sex M
Body weight (g) 386
Date of birth 20081117
Injection date 20170412
Survival period (day) 27
 Tracer 1 (thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
 Direction anterograde
 Method 0.1ul x 2 (@ 0.8 and 1.2mm) 5min each
 Tracer 2 10 % BDA
 Injection Site contra/cingular
 Direction antero
 Method 1ul/10 min

CONTENT

File name Description
NN_tracer/
c2_50mu/
labels/