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Marmoset Tracer Injection – Image set [MTI-R01_0030]
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README.md

Marmoset Tracer-Injection [R01_0030]

Authors: Akiya Watakabe1*, Henrik Skibbe2

  1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
  2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0030

  • Microscopy images and annotations: Akiya Watakabe.
  • Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA
Brain/MINDS ID R01_0030
Group Yamamori
Lab identifier TT23
Region of injection A8aV
Side of injection left
Summary A8aV and A47L; extra-PFC pattern looks more similar to 8aD than 8aV. (8aD-A)
Known issues This sample used CamkII-tTA as the driver unlike other samples. Corpus callossum was severed for this sample, resulting in a slight gap between the two hemispheres, which affected lamina assignment of the cingular regions.
Age 5.3
Sex F
Body weight (g) 395
Date of birth 20110228
Injection date 20160426
Survival period (day) 28
 Tracer 1 (CamKII1.3-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 1)x10e12 vg/ml
 Direction anterograde
 Method 0.1ul x 1 (@ 1mm)/5min

CONTENT

File name Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.