Marmoset Tracer-Injection [R01_0098]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0098

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0098
Group	Yamamori
Lab identifier	TT73
Region of injection	A24b
Side of injection	left
Summary	A24b/c injection. No A24a label. Diffuse and widespread layer 1 innervation in the cingular and insular areas.
Known issues	Labeling along the needle track. A tissue fragment containing the injeection site is displaced into the midline around section 190.
Age	3.3
Sex	M
Body weight (g)	553
Date of birth	20151004
Injection date	20181218
Survival period (day)	30
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.2ul x 1 (@ 3.1 mm) 5min
Tracer 2	AAV2retro-EF1-cre: 1.5x10e12 vg/ml
Injection Site	Coinjection with tracer 1
Direction	retro
Tracer 3	10 % BDA
Injection Site	contra-injection
Direction	antero
Method	0.2µl/5min x1

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.