Marmoset Tracer-Injection [R01_0092]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0092

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0092
Group	Yamamori
Lab identifier	TT64
Region of injection	A24a
Side of injection	left
Summary	A24a injection ("mPFC"). Leak into Cd. No label in A24b.
Known issues	Injection into narrow A24a. No leak into above-lying A24b. But small leak into the striatum (labeling of caudate cells lead to unusual labeling of striate-to SN pathway.) IG (indusium griseum) may be strogly labeled which may innervate septum.
Age	2.4
Sex	M
Body weight (g)	305
Date of birth	20160211
Injection date	20180703
Survival period (day)	27
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.2ul x 1 (@ 4.3 mm) 5min
Tracer 2	AAV2retro-EF1-cre: 1.5x10e12 vg/ml
Injection Site	Coinjection with tracer 1
Direction	retro
Tracer 3	10 % BDA
Injection Site	contra-injection
Direction	antero
Method	0.5µl/5min x1

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.