MTI-R01_0063/README.md

# Marmoset Tracer-Injection [R01_0063]

Authors: Akiya Watakabe<sup>1</sup>*, Henrik Skibbe<sup>2</sup>

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)


## DATASET DESCRIPTION
    
This dataset contains **TissueCyte** images produced during **tracer injection** experiment on Marmoset **R01_0063**

* Microscopy images and annotations: Akiya Watakabe.
* Image post-processing: Henrik Skibbe.


For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection


## EXPERIMENT METADATA
|||
|----|-----|
|Brain/MINDS ID|R01_0063|
|Lab identifier|TT42|
|Region of injection|A8aV|
|Summary|A8aV injection.  A problem in stage position around section 459.  |
|Known issues|Sample came off the stage after section 459.  Due to position shift on comeback, there occured abrupt position shift at section 460 and misregistration in the nearby sections (440-459).  |
|Age|8.3|
|Sex|M|
|Date of birth|20090203|
|Injection date|20170517|
|Survival period (day)|28|
|&#x00A0;Tracer **1** |(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml|
|&#x00A0;Direction|anterograde|
|&#x00A0;Method|0.1ul x 2 (@ 0.8 and 1.2mm) 5min each|
|&#x00A0;Tracer **2** |10 % BDA|
|&#x00A0;Injection Site|contra/parietal|
|&#x00A0;Direction|antero|
|&#x00A0;Method|0.5ul/5minx2|


## CONTENT

| File name | Description |
| ---- | ---- |
|[`NN_tracer`](NN_tracer)/||
|[`c2_50mu`](c2_50mu)/||
|[`labels`](labels)/||
|[`c1_50mu`](c1_50mu)/||
|[`c3_50mu`](c3_50mu)/||
|[`Backlit_slices`](Backlit_slices)/||
|[`Nissl_slices`](Nissl_slices)/||
initial release 2021-12-27 00:00:00 +09:00			`# Marmoset Tracer-Injection [R01_0063]`

			`Authors: Akiya Watakabe<sup>1</sup>*, Henrik Skibbe<sup>2</sup>`

			`1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science`
			`2. Brain Image Analysis Unit, RIKEN Center for Brain Science`

			`*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)`


			`## DATASET DESCRIPTION`

			`This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0063`

			`* Microscopy images and annotations: Akiya Watakabe.`
			`* Image post-processing: Henrik Skibbe.`


			`For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection`


			`## EXPERIMENT METADATA`
			`\|\|\|`
			`\|----\|-----\|`
			`\|Brain/MINDS ID\|R01_0063\|`
			`\|Lab identifier\|TT42\|`
			`\|Region of injection\|A8aV\|`
			`\|Summary\|A8aV injection. A problem in stage position around section 459. \|`
			`\|Known issues\|Sample came off the stage after section 459. Due to position shift on comeback, there occured abrupt position shift at section 460 and misregistration in the nearby sections (440-459). \|`
			`\|Age\|8.3\|`
			`\|Sex\|M\|`
			`\|Date of birth\|20090203\|`
			`\|Injection date\|20170517\|`
			`\|Survival period (day)\|28\|`
			`\| Tracer 1 \|(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml\|`
			`\| Direction\|anterograde\|`
			`\| Method\|0.1ul x 2 (@ 0.8 and 1.2mm) 5min each\|`
			`\| Tracer 2 \|10 % BDA\|`
			`\| Injection Site\|contra/parietal\|`
			`\| Direction\|antero\|`
			`\| Method\|0.5ul/5minx2\|`


			`## CONTENT`

			`\| File name \| Description \|`
			`\| ---- \| ---- \|`
			\|[`NN_tracer`](NN_tracer)/\|\|
			\|[`c2_50mu`](c2_50mu)/\|\|
			\|[`labels`](labels)/\|\|
add c1, c3, backlit & nissl slices 2023-08-17 00:00:00 +09:00			\|[`c1_50mu`](c1_50mu)/\|\|
			\|[`c3_50mu`](c3_50mu)/\|\|
			\|[`Backlit_slices`](Backlit_slices)/\|\|
			\|[`Nissl_slices`](Nissl_slices)/\|\|