Marmoset Tracer-Injection [R01_0061]

Authors: Akiya Watakabe¹*, Henrik Skibbe²

1. Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science
2. Brain Image Analysis Unit, RIKEN Center for Brain Science

*corresponding authors: Akiya Watakabe (akiya.watakabe@riken.jp)

DATASET DESCRIPTION

This dataset contains TissueCyte images produced during tracer injection experiment on Marmoset R01_0061

• Microscopy images and annotations: Akiya Watakabe.
• Image post-processing: Henrik Skibbe.

For more details see https://dataportal.brainminds.jp/marmoset-tracer-injection

EXPERIMENT METADATA

Brain/MINDS ID	R01_0061
Group	Yamamori
Lab identifier	TT40
Region of injection	A8b
Side of injection	left
Summary	A8b injection with A6M leak. Columnar projections are rare. Robust deep layer projection in PCC.
Known issues	Targeted to A8b, but leaked into A6M. Injection was almost restricted to deep layers.
Age	8.5
Sex	M
Body weight (g)	386
Date of birth	20081117
Injection date	20170412
Survival period (day)	27
Tracer 1	(thy1-tTA 1/TRE-clover 1/TRE-Vamp2mPFC 0.25)x10e12 vg/ml
Direction	anterograde
Method	0.1ul x 2 (@ 0.8 and 1.2mm) 5min each
Tracer 2	10 % BDA
Injection Site	contra/cingular
Direction	antero
Method	1ul/10 min

CONTENT

File name	Description
NN_tracer/
c2_50mu/
labels/
c1_50mu/
c3_50mu/
Backlit_slices/
Nissl_slices/

Note: Color lookup table used in atlas region labels slices can be found here.